EVALUATING SPERM DNA FRAGMENTATION INDEX USING TUNEL ASSAY FORUNEXPLAINED INFERTILITY: ITS ASSOCIATION WITH DEMOGRAPHIC ANDLIFESTYLE FACTORS

Abstract

Infertility has been affecting globally, influenced by both men and women factors. Evaluation of
female factor and male factor using traditional semen analysis were carried out on the routine
basis. In our study findings, we observed males with normal semen analysis report have abnormal
readings of DNA fragmentation index (more than 12%).We assessed the DNA integrity using
TUNEL assay by measuring the sperm DFI using flow cytometry. Demographic and lifestyle
information including age, smoking habit, alcohol consumption, occupational status, educational
profile, clothing type and dietary factors were noted. It was observed that smoking, alcohol and
non-vegetarian diet were associated with increased values of DNA fragmentation (>12%). Out of
total 110 subjects, 51% were having normal values of DFI whereas 49% of the subjects showed
high DFI values. Maximum DFI calculated was 42.5%, which was much higher than the threshold
value. The major lifestyle factors discussed in the present study are amongst the potential risk
factors that could impair the male fertility. Thus, greater awareness and recognition provide better
treatment options for those couples seeking conception